From bench to production
Expert consulting for cell line development
Specialized in stable cell line engineering, genetic code expansion, and ncAA-based conjugation systems for ADC production.
Get in Touch
Expert consulting for cell line development
Specialized in stable cell line engineering, genetic code expansion, and ncAA-based conjugation systems for ADC production.
Get in Touch
With a PhD in Cell Biology and over a decade leading research at LMU Munich, I have built deep expertise in stable cell line engineering, gene editing (CRISPR/Cas), and genetic code expansion for ncAA-based conjugation systems.
My work on amber suppression optimization led to iPASS — a tool now used by labs worldwide for designing efficient ncAA incorporation sites.
I combine hands-on bench experience with strong bioinformatics skills, bringing a data-driven approach to cell line development and genome engineering challenges.
Today, I consult for biotech startups working on ADCs, biologics, and next-generation protein engineering. I combine deep technical expertise with a structured, project-management approach — ensuring clear milestones, documentation, and efficient use of your consulting hours.
Design and optimization of integration strategies for high-copy transgene expression. PiggyBac, Bxb1, and targeted integration approaches.
Genetic code expansion for site-specific conjugation. Optimization of amber suppression efficiency, tRNA/synthetase systems, and click chemistry workflows.
Gene editing strategy design, knock-in/knock-out optimization, and integration site selection for mammalian cell lines.
Sequence analysis, codon optimization, integration site prediction, and experimental data analysis for cell line development projects.
Identification of Permissive Amber Suppression Sites — a prediction tool for optimal TAG codon placement in proteins for efficient ncAA incorporation.
Try iPASSIdentification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells
Bartoschek MD, Ugur E, Nguyen TA, Rodschinka G, Wierer M, Lang K, Bultmann S
Nucleic Acids Research (2021) 49(11):e62
A modular open platform for systematic functional studies under physiological conditions
Mulholland CB, Smets M, Schmidtmann E, [...] Bultmann S, Leonhardt H
Nucleic Acids Research (2015) 43(17):e112
Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers
Bultmann S, Morbitzer R, Schmidt CS, [...] Leonhardt H
Nucleic Acids Research (2012) 40(12):5368-77
Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system
Anton T, Bultmann S, Leonhardt H, Markaki Y
Nucleus (2014) 5(2):163-72
Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
Szwagierczak A, Bultmann S, Schmidt CS, Spada F, Leonhardt H
Nucleic Acids Research (2010) 38(19):e181
Have a cell line challenge? Looking for expert input on your ADC pipeline? Get in touch.